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Merck

C3888

Sigma-Aldrich

Carboxypeptidase Y from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥50 units/mg protein

Sinónimos:

Peptidyl-L-amino acid Hydrolase, Serine Carboxypeptidase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54

grado

Proteomics Grade

Nivel de calidad

formulario

lyophilized powder

actividad específica

≥50 units/mg protein

mol peso

61 kDa

composición

Protein, ≥75% E1%/280

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

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Aplicación

Carboxypeptidase Y has a broad specificity and is stable in urea. Hence, this enzyme is not like other carboxypeptidases and can be used for sequence analysis. Due to its amidase action, this enzyme might be applied to the sequence analysis of peptides having amidated COOH-terminal groups such as oxytocin and vasopressin.

Acciones bioquímicas o fisiológicas

The glycoprotein has a molecular weight of about 61,000, has a nitrogen content of 12.74%. It is a single polypeptide chain of 442 residues with 16 residues of glucosamine in the carbohydrate moiety. Lysine is at the NH2 terminus and -Asp-Ser-Thr-Leu is the COOH-terminal sequence. The principal action of the enzyme is to remove COOH-terminal residues from polypeptide chains. It is used as a vacuolar marker enzyme for studies on protein transport and localization.

Envase

Package size based on protein content

Definición de unidad

One unit will hydrolyze 1.0 μmole of N-CBZ-Phe-Ala to N-CBZ-L-phenylalanine and L-alanine per min at pH 6.75 at 25°C, based on EM/230 = 191.5.

Forma física

Lyophilized powder containing citric acid.

Nota de análisis

amidase and esterase activities may be present

Pictogramas

Health hazardExclamation mark

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Caroline A Magalhães et al.
Memorias do Instituto Oswaldo Cruz, 106(2), 146-152 (2011-05-04)
Typical and atypical enteropathogenic Escherichia coli (EPEC) are considered important bacterial causes of diarrhoea. Considering the repertoire of virulence genes, atypical EPEC (aEPEC) is a heterogeneous group, harbouring genes that are found in other diarrheagenic E. coli pathotypes, such as
H R Stennicke et al.
Biochemistry, 35(22), 7131-7141 (1996-06-04)
The activity of serine carboxypeptidases is dependent on a catalytic triad, an oxyanion hole, and a binding site equivalent to those found in the serine endopeptidases. The action of carboxypeptidase Y on substrates containing amino acids, alcohols, and amines as
L Ballou et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(9), 3368-3372 (1990-05-01)
The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiae mnn9 mutant bulk mannoprotein. The results support the view that the mnn
Johanna Rankenberg et al.
Experimental eye research, 210, 108704-108704 (2021-07-25)
Advanced glycation end products (AGEs) accumulate with age in human lens capsules. AGEs in lens capsules potentiate the transforming growth factor beta-2-mediated mesenchymal transition of lens epithelial cells, which suggests that they play a role in posterior capsule opacification after
Václava Bauerová et al.
Canadian journal of microbiology, 58(5), 678-681 (2012-04-17)
Vacuoles play an important role in the physiology of pathogenic Candida spp. However, information on Candida albicans vacuolar enzymes, their properties, and regulation is scarce. Expression of the genes APR1 and CPY1 encoding vacuolar aspartic protease and serine carboxypeptidase, respectively

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