P6675
Prolidase from porcine kidney
lyophilized powder, ≥100 units/mg protein
Sinónimos:
Aminoacyl-L-proline hydrolase, Imido Dipeptidase, Prolidase, Proline dipeptidase
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About This Item
formulario
lyophilized powder
Nivel de calidad
actividad específica
≥100 units/mg protein
composición
Protein, 20-74% Lowry
temp. de almacenamiento
−20°C
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Categorías relacionadas
Descripción general
Prolidase is a cytosolic exopeptidase. It is a homodimeric enzyme which requires divalent cations like manganese as a cofactor in its active site for its function.
Aplicación
Prolidase from porcine kidney has been used:
- in the enzymatic hydrolysis of porcine milk for the recovery of L-glutamine from proteins and peptides
- in the proteolysis of skim milk for the determination of ε-(γ-glutamyl)lysine and free aminoacids
- to determine its effect on the activity of enterococcin A 2000
Prolidase has an important role in recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins.
Acciones bioquímicas o fisiológicas
Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.
Rare mutation in prolidase gene causes deficiency leading to massive imidodipeptiduria, elevated proline-containing dipeptides in plasma, recurrent infections, mental retardation and skin lesions.
Definición de unidad
One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40 °C.
Forma física
Supplied as a lyophilized powder containing Tris buffer salt and MnCl2.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Órganos de actuación
Respiratory system
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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We found no data in the literature related to oxidative stress index (OSI), total oxidative status (TOS) and prolidase activity in patients with diabetic neuropathy (DN). In this study, we aimed to evaluate the oxidative status of DN patients via
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A group of 23-29-kDa polypeptides in the membranes of bovine rod outer segments are substrates for S-adenosylmethionine-dependent methylation reactions. The bulk of the methyl group incorporation is in base-labile ester-like linkages, and does not appear to be due to the
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