D7656
Deoxyribonucleic acid, single stranded from salmon testes
For hybridization
Sinónimos:
single-stranded template DNA
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
grado
for molecular biology
Nivel de calidad
Ensayo
9-11 mg/mL
Formulario
solution
concentración
10.0 ± 1.0 mg/mL in H2O
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
¿Está buscando productos similares? Visita Guía de comparación de productos
Descripción general
Deoxyribonucleic acid, single stranded from salmon testes is a ready-to-use solution of high quality, sonicated, single-stranded template DNA isolated from the testes of salmon.
Aplicación
Deoxyribonucleic acid, single stranded from salmon testes has been used for/as:
- in situ hybridization histochemistry of rat brain, human kidney sections and renal biopsy tissues
- preparing standard curve of DNA
- prehybridization solution in southern and northern hybridizations
- dilution of the cDNA
- fluorescent in-situ hybridization (FISH)
- ChIP analysis
- a standard to analyse the degraded nucleosides of genomic DNA by high performance liquid chromatography
- a component of hybridization solution in FISH
- a control in DNA-DNA hybridization experiment between various bacterial species
- to precipitate probes in fluorescence in situ hybridization (FISH)
For use as a blocking agent in Northern and Southern blotting.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Suitable for use as a blocking agent in Southern hybridizations.
Nota de preparación
This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Otras notas
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Jeffrey A Hodson et al.
Nucleic acids research, 31(21), e134-e134 (2003-10-25)
In this paper we report the construction of a Schizosaccharomyces pombe strain that facilitates analysis of replicating DNA. The strain co-expresses the Herpes simplex virus thymidine kinase gene (hsv-tk) and a human equilibrative nucleoside transporter (hENT1). The double integrant efficiently
Similar rates of chromosomal aberrant secondary oocytes in two indigenous cattle (Bos taurus) breeds as determined by dual-color FISH
Pauciullo, Alfredo, et al
Theriogenology, 675-683 (2012)
Frequency of aneuploidy in in vitro-matured MII oocytes and corresponding first polar bodies in two dairy cattle (Bos taurus) breeds as determined by dual-color fluorescent in situ hybridization
Nicodemo, D., et al
Theriogenology, 523-529 (2010)
L Kölby et al.
British journal of cancer, 89(7), 1383-1388 (2003-10-02)
The radio-iodinated noradrenaline analogue meta-iodobenzylguanidine (MIBG) can be used for scintigraphy and radiation therapy of neuroendocrine (NE). The aim of the present study was to study the importance of vesicular monoamine transporters (VMATs) for the uptake of (123)I-MIBG in NE
I Shibuya et al.
The Journal of physiology, 514 ( Pt 2), 351-367 (1998-12-16)
1. The expression, distribution and function of P2X purinoceptors in the supraoptic nucleus (SON) were investigated by reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization, and Ca2+-imaging and whole-cell patch-clamp techniques, respectively. 2. RT-PCR analysis of all seven known P2X
Artículos
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico