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Key Documents

A4781

Sigma-Aldrich

Asialofetuin from fetal calf serum

Type I (Sigma designation)

Sinónimos:

Asialofetuin

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About This Item

Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.61

origen biológico

bovine (calf) serum

Nivel de calidad

tpo

Type I (Sigma designation)

formulario

powder

impurezas

salt, essentially free
≤0.5% N-acetylneuraminic acid

solubilidad

0.85% sodium chloride: soluble 1 mg/mL

temp. de almacenamiento

2-8°C

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Descripción general

Asialofetuin is a glycoprotein with three asparagine-linked triantennary complex carbohydrate chains and terminal N-acetylgalactosamine residues.

Aplicación

Asialofetuin from fetal calf serum has been used:
  • to quantitate the plant Ricin toxin′s B subunit (RTB) lectin activity in β-phaseolin signal peptide (P)–proinsulin gene (INS)–RTB plants by enzyme-linked immunosorbent assay (ELISA)
  • quantitate VP7:RTB fusion protein in transformed potato tissues by ELISA
  • as a glycoprotein substrate to measure the receptor-binding activity of recombinant RTB and NSP490–RTB fusion proteins
  • to study the nature of the interaction between ferritin and the placenta
Incorporation of asialofetuin (AF) in liposomes strongly enhance delivery to and endocytosis by cells displaying the AF receptor, notably hepatocytes. This provides a very efficient route for gene therapy.

Acciones bioquímicas o fisiológicas

Asialofetuin exhibits affinity to asialoglycoprotein receptor (ASGP-R) on hepatocytes and uses the receptor to enter the cell. This property allows asialofetuin to be used as a ligand to deliver drugs to hepatocytes and as a competitive inhibitor to ASGP-R.

Nota de preparación

Prepared by a modification of Spiro, R.G., J. Biol. Chem., 235, 2860 (1960).

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


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Keiichi Motoyama et al.
Journal of drug delivery, 2011, 476137-476137 (2011-04-15)
The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed
R D Lamparelli et al.
British journal of haematology, 72(1), 100-105 (1989-05-01)
The organ distribution of intravenously injected hepatic ferritin either labelled with 59Fe or with 59Fe and 125I, was studied in pregnant guinea-pigs. At 5 h 71.2% of injected 59Fe was present in the placenta and fetus. Transfer of 59Fe to
Nida Zaidi et al.
International journal of biological macromolecules, 103, 65-73 (2017-05-13)
The SDS-glycoprotein system is mimic of membrane protein-lipid system. Fate of glycoprotein, conformation and the interactive forces involved in membrane milieu are expected to be decided by the net charge on glycoprotein that may change during acidic environment in a
Jakob Meier-Credo et al.
Journal of the American Society for Mass Spectrometry, 33(7), 1293-1302 (2022-06-28)
Identification and sequence determination by mass spectrometry have become routine analyses for soluble proteins. Membrane proteins, however, remain challenging targets due to their hydrophobicity and poor annotation. In particular small membrane proteins often remain unnoticed as they are largely inaccessible
Nak-Won Choi et al.
Journal of biotechnology, 121(2), 272-283 (2005-09-27)
The castor-oil plant Ricinus communis A-B dimeric toxin B subunit (RTB) was genetically linked at its N-terminus with a 90 amino acid peptide from simian rotavirus SA-11 non-structural protein NSP4(90) and produced in Escherichia coli BL21 cells. Biologically active recombinant

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