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88793

Sigma-Aldrich

Atto 532 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sinónimos:

Atto 532

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About This Item

Número MDL:
MFCD05865401
Código UNSPSC:
12352108
NACRES:
NA.32

Línea del producto

BioReagent

Ensayo

≥90% (HPLC)
≥90% (degree of coupling)

Formulario

powder

fabricante / nombre comercial

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

Absorción UV

λ: 532-538 nm Amax

idoneidad

suitable for fluorescence

temp. de almacenamiento

−20°C

Descripción general

Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser.

Aplicación

Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.

Características y beneficios

Characteristic features of the Atto 532 NHS ester are:
  • Strong Absorption.
  • High Fluorescence quantum yield.
  • High Photostability.
  • Excellent water solubility.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
BCCK6846
BCCK3352
BCCJ9358
BCCJ0134
BCCH8479

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Rumelo Amor et al.
Scientific reports, 4, 7359-7359 (2014-12-09)
Standing-wave excitation of fluorescence is highly desirable in optical microscopy because it improves the axial resolution. We demonstrate here that multiplanar excitation of fluorescence by a standing wave can be produced in a single-spot laser scanning microscope by placing a
Roland Bienert et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 12(3), 510-517 (2011-02-03)
H(+)-ATP synthases are molecular machines which couple transmembrane proton transport with ATP synthesis from ADP and inorganic phosphate by a rotational mechanism. Single-pair fluorescence resonance energy transfer (spFRET) in single molecules is a powerful tool to analyse conformational changes. It
Wonchul Shin et al.
Cell, 173(4), 934-945 (2018-04-03)
Fusion is thought to open a pore to release vesicular cargoes vital for many biological processes, including exocytosis, intracellular trafficking, fertilization, and viral entry. However, fusion pores have not been observed and thus proved in live cells. Its regulatory mechanisms and
Andrea Armbrüster et al.
FEBS letters, 579(9), 1961-1967 (2005-03-29)
The ability of subunit C of eukaryotic V-ATPases to bind ADP and ATP is demonstrated by photoaffinity labeling and fluorescence correlation spectroscopy (FCS). Quantitation of the photoaffinity and the FCS data indicate that the ATP-analogues bind more weakly to subunit
Daniel Aquino et al.
Nature methods, 8(4), 353-359 (2011-03-15)
We demonstrate three-dimensional (3D) super-resolution imaging of stochastically switched fluorophores distributed across whole cells. By evaluating the higher moments of the diffraction spot provided by a 4Pi detection scheme, single markers can be simultaneously localized with <10 nm precision in
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