72464
Atto 565 NHS ester
BioReagent, suitable for fluorescence, ≥90% (HPLC)
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About This Item
Fórmula empírica (notación de Hill):
C35H34ClN3O11
Peso molecular:
708.11
Número MDL:
Código UNSPSC:
12352108
NACRES:
NA.32
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Línea del producto
BioReagent
Nivel de calidad
Ensayo
≥90% (HPLC)
≥90% (degree of coupling)
Formulario
powder
fabricante / nombre comercial
ATTO-TEC GmbH
idoneidad
suitable for fluorescence
método de detección
fluorometric
temp. de almacenamiento
−20°C
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Descripción general
Atto 565 NHS ester is a commonly used amine-reactive reagent, provided by ATTO-TEC. It is widely used in protein labeling and readily reacts with compounds containing amino groups, forming a chemically stable amide bond between the dye, e.g., a protein. The optimum pH-range for NHS-ester coupling is pH 8.0 – 9.0. At this pH amino groups of proteins, i.e., the ε-amino groups of lysines are unprotonated to a degree sufficiently high for fast coupling.
Características y beneficios
- Strong absorption
- High fluorescence quantum yield
- High thermal and photo-stability
Otras notas
Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.
Información legal
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Artículos
Fluorescent Labeling of Peptides
Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.
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