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Merck

16805

Sigma-Aldrich

Atto 425 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sinónimos:

Atto 425-N-hydroxysuccinimide ester

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About This Item

Fórmula empírica (notación de Hill):
C26H30N2O8
Número de CAS:
Peso molecular:
498.53
Número MDL:
Código UNSPSC:
12352108
ID de la sustancia en PubChem:
NACRES:
NA.32

Línea del producto

BioReagent

Nivel de calidad

Análisis

≥90% (HPLC)
≥90% (degree of coupling)

fabricante / nombre comercial

ATTO-TEC GmbH

solubilidad

DMF: soluble
DMSO: soluble
H2O: soluble

λ

in ethanol (with 0.1% trifluoroacetic acid)

Absorción UV

λ: 422-428 nm Amax

idoneidad

suitable for fluorescence

temp. de almacenamiento

−20°C

cadena SMILES

CCOC(=O)C1=Cc2cc3C(C)CC(C)(C)N(CCCC(=O)ON4C(=O)CCC4=O)c3cc2OC1=O

InChI

1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3

Clave InChI

PUEQEMDTFPYCDY-UHFFFAOYSA-N

Descripción general

Atto 425 is a member of the commercially available coumarin derivatives.

Aplicación

Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .
Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Otras notas

Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS; employed in triple-color coincidence analysis.

Información legal

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Microcontact printing (mCP) is used to immobilize dyes and peptides asymmetrically, by a "peptide coupling" reaction, on monolayers of zeolite L crystals in the contact area between the stamp and the surface of the monolayer. Chemically patterned surfaces of monolayers

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