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Sigma-Aldrich

Atto 488 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sinónimos:

Atto 488

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About This Item

Código UNSPSC:
12352108
NACRES:
NA.32

Línea del producto

BioReagent

Análisis

≥90% (HPLC)
≥90% (degree of coupling)

fabricante / nombre comercial

ATTO-TEC GmbH

λ

in methanol: water (1:1) (with 0.1% perchloric acid)

Absorción UV

λ: 501-507 nm Amax

idoneidad

suitable for fluorescence

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Atto 488 NHS ester is a hydrophilic fluorescent label with high water solubility. The fluorescence activity is excited efficiently at the 480-515nm range. A suitable excitation source for Atto 488 is the 488 nm line of the Argon-Ion laser.

Aplicación

Atto 488 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.

Características y beneficios

Characteristic features of the Atto 488 NHS ester are:
  • Strong Absorption.
  • High Fluorescence quantum yield.
  • High Photostability.
  • Excellent water solubility.

Información legal

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Analysis of fluorescent nanostructures in biological systems by means of spectral position determination microscopy (SPDM).
Muller, P., et al. et al.
Current Microscopy Contributions to Advances in Science and Technology, 1, 3-12 (2012)
Qian Peter Su et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(26), 15036-15046 (2020-06-17)
Mammalian DNA replication is initiated at numerous replication origins, which are clustered into thousands of replication domains (RDs) across the genome. However, it remains unclear whether the replication origins within each RD are activated stochastically or preferentially near certain chromatin
Switching modulation for protein labeling with activatable fluorescent probes.
Kalyan K Sadhu et al.
Chembiochem : a European journal of chemical biology, 12(9), 1299-1308 (2011-06-02)
Christiane Iserman et al.
Molecular cell, 80(6), 1078-1091 (2020-12-09)
We report that the SARS-CoV-2 nucleocapsid protein (N-protein) undergoes liquid-liquid phase separation (LLPS) with viral RNA. N-protein condenses with specific RNA genomic elements under physiological buffer conditions and condensation is enhanced at human body temperatures (33°C and 37°C) and reduced
Gražvydas Lukinavičius et al.
Current opinion in chemical biology, 15(6), 768-774 (2011-11-15)
Numerous synthetic fluorophores have been developed that can switch their spectroscopic properties upon interaction with other molecules or by irradiation with light. In recent years, protein-labeling techniques have been introduced that permit the specific attachment of such molecules to proteins
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