ROAMYGL

Amyloglucosidase

from Aspergillus niger

• Sinónimos: Glucoamylase, disaccharidase-type-α-D-glucosidase
• Comisión internacional de enzimas: 3.2.1.3 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: Aspergillus niger
• Nivel de calidad: 100
• formulario: suspension
• actividad específica: ~14 units/mg protein (At 25 °C with glycogen as the substrate; standardized with BSA.)
• mol peso: M_r 97 kDa
• envase: pkg of 10 mL (10102857001 [100 mg])
• fabricante / nombre comercial: Roche
• Parámetros: 55 °C optimum reaction temp.
• pH óptimo: 4.6-4.8
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Amyloglucosidase is synthesized by several Aspergillus genus species. It is a disaccharidase–type α-glucosidase. This enzyme is an exo-enzyme and one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Especificidad

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.
Heat inactivation: Heat inactivation is recommended at 80 °C for 45 minutes, followed by rapidly cooling down.

Aplicación

Amyloglucosidase from Aspergillus niger can be used for the hydrolyzation of terminal α1,4- and α1,6-glucosidic bonds (glucose-glucose bonds) in polysaccharides (e.g., starch, dextrins, glycogen), removing glucose units sequentially from the non-reducing end of the molecule. The enzyme will also cleave maltose and maltosides (maltotriose, maltotetraose, etc.).

Acciones bioquímicas o fisiológicas

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry for the commercial production of D-glucose from corn syrups.

Definición de unidad

Unit Conversion: One unit (+25 °C; glycogen as substrate) corresponds to 8.6 U (+60 °C; starch as substrate).

Forma física

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash