PGI-RO

Phosphoglucose Isomerase (PGI)

from yeast

• Sinónimos: glucose-6-phosphate isomerase
• Comisión internacional de enzimas: 5.3.1.9 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: yeast
• Nivel de calidad: 100
• Formulario: suspension
• actividad específica: ~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)
• envase: pkg of 1 mL (10127396001 [2 mg]); pkg of 1 mL (10128139001 [10 mg])
• fabricante / nombre comercial: Roche
• pH óptimo: 7.0-7.6
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.

Especificidad

Phosphoglucose isomerase (PGI) is specific for G6P (K_m = 0.7 mM) and fructose-6-phosphate (F6P).

Aplicación

Isomerization of ketoses to aldoses.

Calidad

Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase

Forma física

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Nota de preparación

Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): No data available
• Punto de inflamabilidad (°C): No data available