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11388908910

Roche

Biotin-16-UTP

pkg of 25 μL (250 nmol; 10mM)

Sinónimos:

Biotin-16-UTP, biotin

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About This Item

Código UNSPSC:
41116100

Análisis

97.7% (HPLC)

Nivel de calidad

100

formulario

solution

mol peso

Mr 987.5 (biotin-16-UTP-Li4)

envase

pkg of 25 μL (250 nmol; 10mM)

fabricante / nombre comercial

Roche

color

colorless

solubilidad

water: miscible

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Aplicación

Biotin-labeled RNA can be used as a hybridization probe for:
  • Northern blots
  • Southern blots
  • Plaque or colony lifts
  • RNase protection experiments
  • In situ hybridization
  • Microarray hybridization
The labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

Calidad

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).

Forma física

Biotin-16-UTP, tetralithium salt, 10mM solution

Otras notas

For life science research only. Not for use in diagnostic procedures.

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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