11685619910
Roche
Fluorescein RNA Labeling Mix
solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization
Sinónimos:
RNA labeling
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About This Item
Código UNSPSC:
41105500
Productos recomendados
formulario
solution
Nivel de calidad
uso
sufficient for 20 reactions
envase
pkg of 40 μL
fabricante / nombre comercial
Roche
técnicas
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
temp. de almacenamiento
−20°C
Descripción general
Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).
Especificidad
Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).
Aplicación
Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
- in situ hybridization to tissues
- Southern blots
- northern blots
- plaque or colony lifts
- RNase protection experiments
Calidad
The mix is function tested in combination with the DIG RNA Labeling Kit.
Principio
Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.
Nota de preparación
10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
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