DIGUTP-RO
Roche
Digoxigenin-11-UTP
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Código UNSPSC:
41105500
NACRES:
NA.21
Productos recomendados
Ensayo
≥85% (HPLC)
Nivel de calidad
Formulario
solution
mol peso
(Mr = 1106.7 (DIG-11-UTP-Li4))
envase
pkg of 25 μL (11209256910 [10 mM])
pkg of 57 μL (03359247910 [3.5 mM])
fabricante / nombre comercial
Roche
temp. de almacenamiento
−20°C
Categorías relacionadas
Descripción general
Digoxigenin (DIG) -11-uridine triphosphate (UTP) is provided as a solution of the tetralithium salt in 3.5mM (200nmol) or 10mM (250nmol) concentration. It is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for DIG labeling of RNA in a ratio of 35:65%.
Aplicación
Digoxigenin-11-UTP has been used for labeling RNA probes in:
- in situ hybridization
- in situ reverse transcriptase (RT)-polymerase chain reaction (PCR)
- fluorescence in situ hybridization (FISH)
Acciones bioquímicas o fisiológicas
Linearized template DNA with T7, SP6, or T3 promoter can be in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and digoxigenin-11-UTP, respectively. The labeled RNA can be subsequently detected with the anti-Digoxigenin-AP (alkaline phosphatase), fab fragments, the digoxigenin nucleic acid detection kit, or the digoxigenin luminescent detection kit for nucleic acids.
Calidad
Typical analysis: 85% DIG-11-UTP (HPLC, area%)
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Function tested with the DIG RNA Labeling Kit.
Formula: C43H61N4O22P3Li4
Otras notas
For life science research only. Not for use in diagnostic procedures.
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral
Código de clase de almacenamiento
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Isolation and functional characterization of two dioxygenases putatively involved in bixin biosynthesis in annatto (Bixa orellana L.)
<BIG>Carballo U, et al.</BIG>
PeerJ, 7 (2019)
Félix Legendre et al.
Journal of visualized experiments : JoVE, (71)(71), e50057-e50057 (2013-02-15)
Assessing the expression pattern of a gene, as well as the subcellular localization properties of its transcribed RNA, are key features for understanding its biological function during development. RNA in situ hybridization (RNA-ISH) is a powerful method used for visualizing
Chromatin in situ proximity (ChrISP): single-cell analysis of chromatin proximities at a high resolution
<BIG>Chen X, et al.</BIG>
Biotechniques, 56 (2014)
Studying gene expression in whole embryos by in situ hybridization: A peer-to-peer laboratory guide
<BIG>Kalra A and Xia D</BIG>
Arsenal (2017)
A George et al.
Connective tissue research, 33(1-3), 67-72 (1995-01-01)
Acidic phosphorylated proteins are prominent constituents of the extracellular matrix of bone and dentin. It has been postulated that they may have important structural and regulatory roles in the process of tissue mineralization. Studies of a cDNA library, prepared from
Global Trade Item Number
| Número de referencia del producto (SKU) | GTIN |
|---|---|
| 3359247910 | 4061826742723 |
| 11209256910 | 4061837902062 |
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico