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11333089001

Roche

Anti-Digoxigenin

from sheep

Sinónimos:

anti-digoxigenin, digoxigenin

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About This Item

Código UNSPSC:
12352203

origen biológico

sheep

Nivel de calidad

100

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

lyophilized

envase

pkg of 200 μg

fabricante / nombre comercial

Roche

isotipo

IgG

temp. de almacenamiento

2-8°C

Descripción general

Digoxigenin is a hapten which is used in labeling of nucleic acids and in detection systems.
Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes. It allows faster detection, is less hazardous and has an increased shelf life.

Especificidad

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Aplicación

Anti-Digoxigenin has been used in DNA tethering. It has been used to attach DNA molecule to the glass surface of the flow cell.
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled compounds using:
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot

Acciones bioquímicas o fisiológicas

In the presence of Na+, Mg2+ and ATP, digoxigenin inhibits sodium pumps.

Nota de preparación

Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: for coating: 2 to 4 μg/ml
  • Immunohistocytochemistry: 0.5 to 2 μg/ml
  • In situ hybridization: 0.2 to 0.4 μg/ml
  • Western blot: 0.5 to 2 μg/ml

Working solution: For coating applications: PBS (phosphate buffered saline), pH 7.4
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption.

Reconstitución

Add 1 ml PBS to a final concentration of 200 μg/ml.

Nota de análisis

No cross reactivity with other steroids, such as human estrogens e.g., estradiol or androgens e.g., testosterone.

Otras notas

For life science research only. Not for use in diagnostic procedures.

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Pictogramas

Exclamation mark
GHS07

Palabra de señalización

Warning

Frases de peligro

H317

Clasificaciones de peligro

Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

does not flash

Punto de inflamabilidad (°C)

does not flash

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Affinity Labeling of a Sulfhydryl Group in the Cardiacglycoside Receptor Site of Na+/K+?ATPase by N?Hydroxysuccinimidyl Derivatives of Digoxigenin.
Antolovic R, et al.
European Journal of Biochemistry, 227(1?2), 61-67 (1995)
Alistair Nicol Boettiger et al.
Cell reports, 3(1), 8-15 (2013-01-29)
Transcription is commonly held to be a highly stochastic process, resulting in considerable heterogeneity of gene expression among the different cells in a population. Here, we employ quantitative in situ hybridization methods coupled with high-resolution imaging assays to measure the
Methods in Molecular Biology (2011)
Electron paramagnetic resonance of a single NV nanodiamond attached to an individual biomolecule.
Teeling-Smith R M, et al.
Biophysical Journal, 110(9), 2044-2052 (2016)
Jonathan Silver et al.
Biosensors & bioelectronics, 63, 117-123 (2014-07-30)
We describe a proof-of-principle, immune sandwich assay in which immune complexes link micron-size beads via DNA tethers to a sensor surface. The number of tethered beads, counted using low-magnification microscopy, provides a measure of the concentration of analyte. The prototype
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Digoxigenin (DIG) Labeling Methods

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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