11333062910
Roche
Anti-Digoxigenin
from mouse IgG1κ (clone 1.71.256)
Sinónimos:
anti-digoxigenin, digoxigenin
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About This Item
Código UNSPSC:
12352203
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
clone 1.71.256, monoclonal
Formulario
lyophilized
envase
pkg of 100 μg
fabricante / nombre comercial
Roche
técnicas
ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL
isotipo
IgG1κ
temp. de almacenamiento
2-8°C
Descripción general
Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.
Especificidad
The monoclonal antibody reacts with free and bound digoxigenin.
Aplicación
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
- Immunofluorescence staining
- FISH (fluorescent in situ hybridization)
Acciones bioquímicas o fisiológicas
In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.
Características y beneficios
- Specific to free and bound digoxigenin
- The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.
Nota de preparación
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: For coating applications
phosphate buffered saline, pH 7.4
- ELISA: for coating: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications
phosphate buffered saline, pH 7.4
Reconstitución
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Almacenamiento y estabilidad
The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.
Nota de análisis
There are no cross reactivities known.
Otras notas
For life science research only. Not for use in diagnostic procedures.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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Artículos
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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