11333054001

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester

>90% (elementary analysis), pkg of 5 mg, powder, pH range 2-13

• Sinónimos: Digoxigenin NHS-ester, dig-nhs-ester, ε-(Digoxigenin-3-0-acetamido)caproic acid N-hydroxysuccinimide ester, DIG NHS
• Fórmula empírica (notación de Hill): C₃₅H₅₀N₂O₁₀
• Número de CAS: 129273-26-3
• Peso molecular: 658.78
• Número MDL: MFCD02093394
• Código UNSPSC: 41105600
• ID de la sustancia en PubChem: 329749387

Propiedades

• Nivel de calidad: 100
• Análisis: >90% (elementary analysis)
• formulario: powder
• mol peso: M_r 658.8
• envase: pkg of 5 mg
• fabricante / nombre comercial: Roche
• intervalo de pH: 2-13
• solubilidad: DMF: soluble; DMSO: soluble; ethanol: soluble
• temp. de almacenamiento: −20°C
• cadena SMILES: [H][C@]12CC[C@]3([H])[C@]([H])(C[C@@H](O)[C@]4(C)[C@H](CC[C@]34O)C5=CC(=O)OC5)[C@@]1(C)CC[C@@H](C2)OCC(=O)NCCCCCC(=O)ON6C(=O)CCC6=O
• InChI: 1S/C35H50N2O10/c1-33-13-11-23(45-20-28(39)36-15-5-3-4-6-31(42)47-37-29(40)9-10-30(37)41)17-22(33)7-8-25-26(33)18-27(38)34(2)24(12-14-35(25,34)44)21-16-32(43)46-19-21/h16,22-27,38,44H,3-15,17-20H2,1-2H3,(H,36,39)/t22-,23+,24-,25-,26+,27-,33+,34+,35+/m1/s1
• Clave InChI: KHNDABJZSPPYLE-FUGFVFQCSA-N

Descripción

Descripción general

Chemical reagent for the labeling of proteins and oligonucleotides. One reactive group is able to interact with NH₂-groups (of proteins or oligonucleotides), another reactive group (Digoxigenin) reacts with Anti-Digoxigenin and thus can be detected.

Aplicación

Digoxigenin-3-O-methylcarbonyl-ε-aminocaproic acid-N-hydroxysuccinimide ester has been used to label the sequence (III) for the linker molecule sequence (VIII).

Acciones bioquímicas o fisiológicas

Digoxigenin, a non-radioactive immune-tag is mainly used for labeling proteins and in situ hybridization.

Principio

The activated ester reacts with amino groups under mild conditions. The digoxigenin moiety can be introduced into proteins or 5′-amino substituted oligonucleotides.

Nota de preparación

Working concentration: 1 to 5 mM
Working solution: Preparation of working solution

Under stirring 0.327 mg digoxigenin-3-O-methylcarbonyl- ε-aminocaproic acid -N-hydroxysuccinimide ester is dissolved in 8.18 μl DMSO or ethanol.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Pictogramas: GHS06,GHS08,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H300,H319,H331,H373,H410
• Consejos de prudencia: P260 - P264 - P273 - P301 + P310 + P330 - P391 - P403 + P233
• Clasificaciones de peligro: Acute Tox. 2 Oral - Acute Tox. 3 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - STOT RE 2
• Código de clase de almacenamiento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable