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11383213001

Roche

NBT

4-Nitro blue tetrazolium chloride, solution

Sinónimos:

Nitroblue tetrazolium chloride

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About This Item

Código UNSPSC:
41116100

Nivel de calidad

100

Análisis

>95%

formulario

solution

mol peso

Mr 817.7

envase

pkg of 3 mL (300 mg)

fabricante / nombre comercial

Roche

técnicas

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

temp. de almacenamiento

−20°C

Descripción general

NBT is a chromogenic and soluble substance. It is a substrate of alkaline phosphatase (AP) and is converted to an indigo/purple dye on its action.

Aplicación

  • Immunohistocytochemistry
  • Western blot
  • Southern blot
  • Northern blot
  • Colony and plaque hybridization
  • In situ hybridization

Forma física

Solution (100 mg/ml) in 70% dimethylformamide (DMF) (v/v). The color of the NBT solution can vary between yellow and brown depending on the lot used.

Note: The color does not impair the quality or the function of the reagent. In some cases a precipitate may occur which is easily brought into solution by briefly warming the substrate at 37 °C.

Nota de preparación

Working solution: Preparation of staining solution
  • Always prepare this solution fresh shortly before use!For all applications except DIG System: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
  • For DIG System applications: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
  • Note: Do not include MgCl2 in the DIG detection buffer as this might lead to spotty background on the membrane after the detection procedure. Alkaline Phosphatase does not require Mg2+.

Otras notas

For life science research only. Not for use in diagnostic procedures.

Pictogramas

Exclamation markHealth hazard
GHS07,GHS08

Palabra de señalización

Danger

Frases de peligro

H319,H332,H360D

Clasificaciones de peligro

Acute Tox. 4 Inhalation - Eye Irrit. 2 - Repr. 1B

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

167.0 °F

Punto de inflamabilidad (°C)

75 °C

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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NBT Protocol & Troubleshooting

There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

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