P4978

Phosphatase, alkalisch aus Kälberdarm

buffered aqueous glycerol solution

• Synonym(e): CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• CAS-Nummer: 9001-78-9
• EC-Nummer: 3.1.3.1 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00131849
• UNSPSC-Code: 12352204
• eCl@ss: 42010105
• NACRES: NA.53

Eigenschaften

• Qualität: for molecular biology
• Form: buffered aqueous glycerol solution
• Mol-Gew.: ~80 kDa
• Konzentration: ≥10,000 units/mL
• UniProt-Hinterlegungsnummer: P19111
• Fremdaktivität: DNase, RNase, none detected
• Versandbedingung: wet ice
• Lagertemp.: −20°C
• Angaben zum Gen: cow ... ALPI(280993)

Beschreibung

Allgemeine Beschreibung

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Anwendung

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Komponenten

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl₂, and 0.1 mM ZnCl₂, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Einheitendefinition

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Sonstige Hinweise

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable