P4252

Phosphatase, alkalisch aus E. coli

ammonium sulfate suspension, 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)

• Synonym(e): Orthophosphor-Monoesterphosphohydrolase (alkalisches Optimum)
• CAS-Nummer: 9001-78-9
• EC-Nummer: 3.1.3.1 (BRENDA, IUBMB)
• EG-Nummer: 232-631-4
• MDL-Nummer: MFCD00131849
• UNSPSC-Code: 12352204
• eCl@ss: 42010105
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: Escherichia coli
• Qualitätsniveau: 300
• Form: ammonium sulfate suspension
• Spezifische Aktivität: 30-90 units/mg protein (modified Warburg-Christian, in glycine buffer)
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 has been used for bulk digestion of tRNA . Product P4252 is provided in the ratio of 30-90 units/mg of protein.

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required. Product P4252 is an ammonium sulfate suspension in glycine buffer.

Biochem./physiol. Wirkung

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.

Vorsicht

Die Anweisenheit von 0.1% SDS beim Alkalischen Phosphate-Assay inhibiert jegliche Nuklease-Kontamination in der Probe.

Einheitendefinition

Eine Einheit hydrolysiert 1,0 μmol p-Nitrophenylphosphat je Min. bei einem pH-Wert von 10,4 und 37 °C.

Physikalische Form

Suspension in 2.5 M (NH₄)₂SO₄

Hinweis zur Analyse

Die Aktivität bei pH 8.0 und 25 °C ist ungefähr die gleiche wie bei pH 10.4 und 37 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)