GE17-5268-02
Ni Sepharose™ High Performance
Cytiva 17-5268-02, pack of 100 mL
Synonym(e):
High Performance Ni Resin, Ni Sepharose
About This Item
Empfohlene Produkte
Verpackung
pack of 100 mL
Hersteller/Markenname
Cytiva 17-5268-02
Matrix
highly cross-linked 6% agarose
Durchschnittliche Teilgröße
34 μm
cleaning in place
2-14(Ni2+-stripped medium.)
working range
3-12(Ni²⁺-stripped medium.)
Kapazität
≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)
Verwandte Kategorien
Allgemeine Beschreibung
Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.
The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.
Leistungsmerkmale und Vorteile
- High-performance purification of histidine-tagged proteins
- Negligible leakage of Ni2+ ions.
- High binding capacity, at least 40 mg/mL medium
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives
- 34 μm bead size for improved resolution
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
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Artikel
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
Protokolle
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Ni Sepharose High Performance consists of highly cross-linked 6% agarose beads (34 µm) to which a chelating group has been immobilized and subsequently charged with Ni2+ ions.
This page shows how to purify protein A-tagged proteins using Cytiva products.
This protocol shows how to remove histidine tags by enzymatic cleavage using Cytiva products.
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