GE28-9574-99
TALON® Superflow™
Cytiva 28-9574-99, pack of 10 mL
Synonym(e):
TALON Affinity Resin
About This Item
Empfohlene Produkte
ligand
TALON® Superflow Cobalt-IMAC
Verpackung
pack of 10 mL
Hersteller/Markenname
Cytiva 28-9574-99
Matrix
6% cross-linked agarose
Partikelgröße
60-160 μm
cleaning in place
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
Kapazität
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
Lagertemp.
2-8°C
Verwandte Kategorien
Allgemeine Beschreibung
Leistungsmerkmale und Vorteile
- Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
- Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
- Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
- Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Analysenzertifikate (COA)
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Artikel
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
How to optimize purification of histidine-tagged proteins using Cytiva products.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.
Protokolle
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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