GE17-0920-07
IMAC Sepharose™ High Performance
Cytiva 17-0920-07, pack of 100 mL
About This Item
Empfohlene Produkte
Verpackung
pack of 100 mL
Hersteller/Markenname
Cytiva 17-0920-07
Matrix
highly cross-linked 6% agarose
Durchschnittliche Teilgröße
34 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
Arbeitsbereich
3-12
Kapazität
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Allgemeine Beschreibung
Anwendung
Leistungsmerkmale und Vorteile
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
- Conveniently charge with your metal of choice
- 34 μm bead size for high performance purification
- High binding capacity
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Signalwort
Warning
H-Sätze
Lagerklassenschlüssel
3 - Flammable liquids
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumente section.
Wenn Sie Hilfe benötigen, wenden Sie sich bitte an Kundensupport
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Kunden haben sich ebenfalls angesehen
Artikel
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from Cytiva.
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
For optimal conditions for growth, induction, and cell lysis of recombinant histidine-tagged proteins, please refer to established procedures. The following is a general procedure for cell lysis and sample preparation from bacterial cultures. Other established procedures may also work.
How to optimize purification of histidine-tagged proteins using Cytiva products.
Protokolle
Protocol for Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap™ Kit, HiTrap® Chelating HP and Chelating Sepharose® Fast Flow products from Cytiva.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..
Setzen Sie sich mit dem technischen Dienst in Verbindung.