I1408

IMAC-Select Affinity Gel

(1:1 suspension in a 30% ethanol solution)

• UNSPSC-Code: 12352200
• NACRES: NA.56

Eigenschaften

• Qualitätsniveau: 200
• Form: (1:1 suspension in a 30% ethanol solution)
• Haltbarkeit: 1 yr
• Methode(n): affinity chromatography: suitable
• Verunreinigungen: ≤0.08 Metal Contamination (μmol/ml packed gel)
• Matrix: 6% beaded agarose
• Kapazität: 10-30 μmol/mL binding capacity (Cu⁺²)
• Versandbedingung: wet ice
• Lagertemp.: 2-8°C

Beschreibung

Anwendung

An immobilized metal affinity chromatography (IMAC) product that can be widely used for purifying proteins, peptides, and nucleotides of interest, most commonly proteins. IMAC-Select utilizes a non-charged linkage chemistry to connect the proprietary quadridentate chelate to the agarose bead matrix. IMAC-Select Affinity Gel is durable and can capture proteins of interest with an affinity to various metal ions such as nickel, cobalt, iron or gallium. A metal free resin allows researchers to add metal of choice, or to use the resin to remove metal contamination from a protein. IMAC-Select Affinity Gel has been used in the affinity purification of kelch-like ECH-associated protein 1 (KEAP1), Wilms tumor gene on the X chromosome (WTX) and glycoprotein G1 tail protein of the andes virus.

Physikalische Form

1:1 suspension in a 30% ethanol solution

Angaben zur Herstellung

The ethanol used to store the affiinity gel must be removed prior to use to avoid precipitation of buffer salts.The affinity gel is first washed with 1 to 2 volumes of deionized water to remove ethanol. The gel is charged with 2 to 3 volumes of a solution of the desired metal ion at a concentration of 10 mg/mL, and then equilibrated with 3 to 5 volumes of equilibration buffer.

Sonstige Hinweise

Do not allow the affinity gel to remain in any buffer for extended periods of time unless it contains an anti-microbial agent such as 30% ethanol.

Sicherheitshinweise

• Piktogramme: GHS02
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P210 - P233 - P240 - P241 - P242 - P243
• Gefahreneinstufungen: Flam. Liq. 3
• Lagerklassenschlüssel: 3 - Flammable liquids
• WGK: WGK 3
• Flammpunkt (°F): 120.2 °F - closed cup
• Flammpunkt (°C): 49 °C - closed cup
• Persönliche Schutzausrüstung: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter