GE17371201

Ni Sepharose^™ Excel

Cytiva 17371201, 90 μm avg. part. size

• Synonym(e): Ni Sepharose^™ Excel
• UNSPSC-Code: 41106500
• NACRES: NA.56

Eigenschaften

• Hersteller/Markenname: Cytiva 17371201
• Matrix: highly cross-linked 6% agarose
• Durchschnittliche Teilgröße: 90 μm
• cleaning in place: 2-14
• working range: 2-12
• Kapazität: ≥10 mg binding capacity (histidine-tagged protein)

Beschreibung

Allgemeine Beschreibung

)Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6-tagged pure protein (Mr 43 000) in EX-CELL 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.

Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.

EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.

His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.

Convenient prepacked columns:

• HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.
• His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.

Leistungsmerkmale und Vorteile

• Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
• Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
• Retained binding capacity of up to 10 mg/mL resin.
• Simplified sample handling – fewer steps needed compared with conventional workflow.
• Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.

Lagerung und Haltbarkeit

Store at 4 to 30 °C (20% ethanol)

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Sepharose is a trademark of Cytiva

Sicherheitshinweise

• Piktogramme: GHS09
• Signalwort: Warning
• H-Sätze: H226
• P-Sätze: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Lagerklassenschlüssel: 3 - Flammable liquids