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Key Documents

CMC0018

Sigma-Aldrich

OverExpress™ C41(DE3) pLysS Chemically Competent Cells

Escherichia coli, rod shaped

Synonym(e):

Chemically competent cells

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About This Item

UNSPSC-Code:
41106202
NACRES:
NA.85

product name

OverExpress™ C41(DE3) pLysS Chemically Competent Cells, for the highest protein expression

Biologische Quelle

Escherichia coli

Qualität

for molecular biology

Wachstumsmodus

adherent or suspension

Morphologie

rod shaped

Methode(n)

microbiological culture: suitable

Zelltransformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

Versandbedingung

dry ice

Lagertemp.

−70°C

Verwandte Kategorien

Allgemeine Beschreibung

OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)

Leistungsmerkmale und Vorteile

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

Komponenten

  • OverExpress C41(DE3) pLysS chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Rechtliche Hinweise

OverExpress is a trademark of Imaxio, S.A.

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Analysenzertifikate (COA)

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SIG10 Chemically Competent Cells for protein expression and DNA plasmid production

Sigma-Aldrich

CMC0001

SIG10 Chemically Competent Cells

E. coli BL21 Cytiva 27-1542-01, pack of 1 vial

GE27-1542-01

E. coli BL21

Chloramphenicol ≥98% (HPLC)

Sigma-Aldrich

C0378

Chloramphenicol

Myles B C Dillon et al.
ACS chemical biology, 7(7), 1198-1204 (2012-04-18)
Protein arginine methyltransferases (PRMTs) catalyze the posttranslational methylation of arginine using S-adenosylmethionine (SAM) as a methyl-donor. The PRMT family is widely expressed and has been implicated in biological functions such as RNA splicing, transcriptional control, signal transduction, and DNA repair.
Ji-Young Youn et al.
Molecular biology of the cell, 21(17), 3054-3069 (2010-07-09)
BAR domains are protein modules that bind to membranes and promote membrane curvature. One type of BAR domain, the N-BAR domain, contains an additional N-terminal amphipathic helix, which contributes to membrane-binding and bending activities. The only known N-BAR-domain proteins in
Myles B C Dillon et al.
The Journal of biological chemistry, 288(39), 27872-27880 (2013-08-16)
Protein arginine methyltransferase (PRMT) 8 is unique among the PRMTs, as it has a highly restricted tissue expression pattern and an N terminus that contains two automethylation sites and a myristoylation site. PRMTs catalyze the transfer of a methyl group
Neale Harrison et al.
PloS one, 9(11), e113007-e113007 (2014-11-26)
N-acylethanolamines are an important class of lipid signaling molecules found in many species, including the nematode Caenorhabditis elegans (C. elegans) where they are involved in development and adult lifespan. In mammals, the relative activity of the biosynthetic enzyme N-acyl phosphatidylethanolamine-specific

Artikel

Bacterial Transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.

Protokolle

OverExpress™ Chemically Competent Cells

Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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