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BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Synonym(e):

Competent E. coli cells

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About This Item

UNSPSC-Code:
41106202
NACRES:
NA.85

product name

BL21(DE3) Chemically Competent Cells, for protein expression

Biologische Quelle

Escherichia coli

Qualität

for molecular biology

Wachstumsmodus

adherent or suspension

Morphologie

rod shaped

Methode(n)

microbiological culture: suitable

Zelltransformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

Versandbedingung

dry ice

Lagertemp.

−70°C

Allgemeine Beschreibung

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Anwendung

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.

Leistungsmerkmale und Vorteile

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Komponenten

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

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Lagerklassenschlüssel

10 - Combustible liquids

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Artikel

Bacterial Transformation

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.

Verwandter Inhalt

BL21 Chemically Competent Cells

BL21 Chemically Competent Cells are provided in aliquots of 80 μL sufficient for two transformation reactions of 40 μL each. Transformation is performed by heat shock at 42 °C, followed by incubation on ice.

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