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CONTROLLER BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.85

product name

CONTROLLER BL21(DE3) Chemically Competent Cells, for controlled protein expression

Biologische Quelle

Escherichia coli

Qualität

for molecular biology

Wachstumsmodus

adherent or suspension

Morphologie

rod shaped

Methode(n)

microbiological culture: suitable

Zelltransformation

competent cell type: chemically competent
transformation efficiency: >1 x 107 cfu/μg

Versandbedingung

dry ice

Lagertemp.

−70°C

Allgemeine Beschreibung

The CONTROLLER BL21(DE3) Chemically Competent Cells are an E. coli strain optimized for high efficiency transformation.
They are ideal for cloning and propagation of plasmid clones. They give high yield and high quality plasmid DNA.
Genotype

F- ompT hsdSB (rB- mB-) gal dcm (DE3) Mini-F lacIq1(GentR)

Leistungsmerkmale und Vorteile

  • Reduce leaky expression: The LacI repressor protein binds to the lacO operator to block transcription of downstream genes. In the absence of an induction agent such as lactose sugar or IPTG, target protein expression from the adjacent promoter will be greatly reduced.
  • High protein yields: Both 10G and BL21(DE3)-based strains are noted for their ability to express high levels of protein. For expression from a T7 promoter please select the HI-Control BL21(DE3) strain. This strain also is deficient in proteases, yielding more stable protein preparations. Most other promoters can be used in either HI-Control strain.
  • Terrific efficiency: The simple, reliable heat-shock procedure for chemical transformation gives excellent results from small amounts of plasmid DNA.

Komponenten

  • CONTROLLER BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Lagerklassenschlüssel

10 - Combustible liquids


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Artikel

Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.

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