CHY5S

αα-Chymotrypsin aus Rinderpankreas

≥40 units/mg protein, vial of 5 mg

• Synonym(e): α-chymotrypsin A and B
• CAS-Nummer: 9004-07-3
• EC-Nummer: 3.4.21.1 (BRENDA, IUBMB)
• EG-Nummer: 232-671-2
• MDL-Nummer: MFCD00130481
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: bovine pancreas
• Qualitätsniveau: 200
• Beschreibung: aseptically filled
• Typ: Type IV-S
• Form: solid
• Spezifische Aktivität: ≥40 units/mg protein
• Mol-Gew.: 25 kDa
• Zusammensetzung: protein, ≥85% UV
• Verpackung: vial of 5 mg
• UniProt-Hinterlegungsnummer: P00767
• Lagertemp.: −20°C
• Angaben zum Gen: cow ... CTRB1(618826)

Beschreibung

Anwendung

α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.

α-Chymotrypsin from bovine pancreas has been used:

• as a supplement for the collection of semen into Tris diluent
• as one of the proteases in the analysis of major histocompatibility complex (MHC) class II protease sensitivity assay
• as a component in YEPD broth for biofilm dispersion assay
• in the preparation of chitinase–chymotrypsin–DMSO buffer (CCD buffer) for enzymatic digestion of larvae

The enzyme from Sigma has been used to assess the effect of limited proteolysis with α-chymotrypsin on the sperm penetration.

Biochem./physiol. Wirkung

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg^2+.

Ein Serin-Protease, die Peptidbindungen mit aromatischen oder großen hydrophoben Seitenketten (Tyr, Trp, Phe, Met, Leu) am Carboxylende der Bindung hydrolysiert.

Einheitendefinition

Eine Einheit hydrolysiert 1.0 μmol BTEE pro min bei pH 7.8 und 25 °C.

Hinweis zur Analyse

Protein determined by A1%/280

Sicherheitshinweise

• Piktogramme: GHS08,GHS07,GHS09
• Signalwort: Danger
• H-Sätze: H302,H315,H319,H334,H335,H400
• P-Sätze: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves