C7762

αα-Chymotrypsin aus Rinderpankreas

Type I-S, essentially salt-free, lyophilized powder

• Synonym(e): α-chymotrypsin A and B, alpha-chymotrypsin
• CAS-Nummer: 9004-07-3
• EC-Nummer: 3.4.21.1 (BRENDA, IUBMB)
• EG-Nummer: 232-671-2
• MDL-Nummer: MFCD00130481
• UNSPSC-Code: 12352204
• eCl@ss: 42010112
• NACRES: NA.54

Eigenschaften

• Typ: Type I-S
• Qualitätsniveau: 200
• Form: essentially salt-free, lyophilized powder
• Spezifische Aktivität: ≥40 units/mg protein
• Mol-Gew.: 25 kDa
• Aufgereinigt durch: 3× crystallization
• Löslichkeit: 1 mM HCl: soluble 2.0 mg/mL, clear
• UniProt-Hinterlegungsnummer: P00767
• Lagertemp.: −20°C
• Angaben zum Gen: cow ... CTRB1(618826)

Beschreibung

Anwendung

α-Chymotrypsin from bovine pancreas has been used in a study to investigate protein extraction by Winsor-III microemulsion systems. α-Chymotrypsin from bovine pancreas has also been used in a study to investigate a new specific fullerene-based fluorescent probe for trypsin.

The product has been used to investigate the inhibitory effect of several ether oligomers against the enzyme. It has also been used to cleave pro-phenoloxidase in order to estimate total phenoloxidase in haemolymph. Furthermore, the enzyme has been used as a positive control in chymotrypsin assays using salivary gland and anterior midgut extracts of Deraeocoris nigritulus.

Biochem./physiol. Wirkung

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, as well as 10 mM of Cu²⁺ and Hg²⁺.

Ein Serin-Protease, die Peptidbindungen mit aromatischen oder großen hydrophoben Seitenketten (Tyr, Trp, Phe, Met, Leu) am Carboxylende der Bindung hydrolysiert.

Einheitendefinition

Eine Einheit hydrolysiert 1.0 μmol BTEE pro min bei pH 7.8 und 25 °C.

Angaben zur Herstellung

Prepared free of autolysis products and low molecular weight contaminants.

The powder may be reconstituted in 1 mM HCl at 2 mg/mL concentration to form a clear solution.

Hinweis zur Analyse

Minimum 85% protein

Protein determined by E1%/280

Sonstige Hinweise

Finden Sie mehr Informationen zu Chymotrypsin auf unserer 'Enzyme-Explorer'-Webseite.

Sicherheitshinweise

• Piktogramme: GHS07,GHS08,GHS09
• Signalwort: Danger
• H-Sätze: H302 - H315 - H319 - H334 - H335 - H400
• P-Sätze: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves