EJG

93051803, bovine adrenal gland, Endothelial

• UNSPSC-Code: 41106514

Eigenschaften

• product name: EJG, 93051803
• Biologische Quelle: bovine adrenal gland
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Endothelial
• Produkte: Factor VIII
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Bovine adrenal medulla endothelial

Beschreibung der Zelllinie

The bovine cell line was established from collagenase-dissociated, adrenal medulla tissue. The cells display a capillary endothelium morphology with relatively large cell bodies and produce factor VIII:C. Tests for BVDV have shown that the cell line is producing both detectable BVDV antigens and infectious BVDV.

Anwendung

Blood clotting studies

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Passage cells every 5 to 7 days.

Sonstige Hinweise

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