MDBK Cell Line bovine

90050801, bovine kidney, Epithelial-like

• Synonym(e): MDBK (NBL-1) Cells, Madin Darby Bovine Kidney Cells, NBL-1 Cells
• UNSPSC-Code: 41106514
• eCl@ss: 32190102

Eigenschaften

• product name: MDBK Cell Line bovine, 90050801
• Biologische Quelle: bovine kidney
• Wachstumsmodus: Adherent
• Karyotyp: Modal no. 51; hypodiploid
• Morphologie: Epithelial-like
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Bovine kidney

Beschreibung der Zelllinie

Derived from the kidney of an apparently normal, adult Bos taurus. Also known as MDBK (NBL-1).

Anwendung

MDBK cell line has been used to study the expression of Eimeria bovis-derived antigens on the host cell surface membrane in vitro. It has also been used to study the ubiquitination and degradation of signal transducer and activator of transcription 1 and 2 (STAT1 and STAT2) by the V proteins of the paramyxoviruses simian virus 5 and human parainfluenza virus type 2.

Nährmedium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or 10% Horse Serum (HS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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