Bu (IMR-31)

89051704

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: buffalo lung
• Wachstumsmodus: Adherent
• Karyotyp: 2n = 60
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Buffalo lung

Beschreibung der Zelllinie

Derived from female Buffalo yearling. The cells have a finite life span of a minimum of 30 passages and are susceptible to a range of viruses including HSV, vaccinia, PRV, BVDV, para-influenza 3, bovine enterovirus (M62/4); also IBRV and VSV (Indiana). The cell line produces both detectable BVDV antigens and infectious BVDV.

Anwendung

Virus replication studies

Nährmedium

McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Sonstige Hinweise

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