SBAC

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: bovine adrenal gland (cortex)
• Verpackung: tube of 5 μg 91021201-DNA-5UG; pkg of vial of cells 91021201-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Fibroblast
• Produkte: Not specified
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Bovine adrenal cortex, normal

Beschreibung der Zelllinie

Derived from the fasciculata-reticularis zone of the adrenal cortex taken from three normal adult cows. FGF stimulates cell division. Cells are responsive to ACTH and other steroidogenic agents. Finite lifespan; 60-70 population doublings expected. Studies have indicated that this cell line produces both detectable BVDV antigens and infectious BVDV.

Anwendung

Hormone studies

Nährmedium

Ham′s F12 with Fibroblast Growth Factor (FGF) (crude, 50ng/ml or pure, 500 pg/ml) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Sonstige Hinweise

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