PORTHOS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: Porcine thyroid
• Verpackung: tube of 5 μg 93122323-DNA-5UG; pkg of vial of cells 93122323-1VL
• Wachstumsmodus: Adherent
• Karyotyp: Not specified
• Morphologie: Epithelial
• Produkte: Thyroglobulin, plasminogen activator (u-PA)
• Rezeptoren: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• Methode(n): cell culture | mammalian: suitable
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Porcine thyroid epithelial

Beschreibung der Zelllinie

Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.

Anwendung

Regulation of thyroid cell metabolism

Nährmedium

Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 ⁴ cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Sonstige Hinweise

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