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HPLC Analysis of Cysteine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive)

HPLC Analysis of Cysteine Enantiomers on Astec® CHIROBIOTIC® T (Formic Acid Mobile Phase Additive) application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

12024AST

Colonna HPLC chirale Astec® CHIROBIOTIC® T

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Precolonna

N° Catalogo
Descrizione
Determinazione del prezzo

12100AST

Astec® Chirobiotic® T Chiral HPLC Guard Column

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

C7352

L-cisteina

from non-animal source, BioReagent, suitable for cell culture, ≥98%

C5360

L-cisteina

30089

L-cisteina

BioUltra, ≥98.5% (RT)

30095

D-Cysteine

≥99% (RT)

861677

DL-Cysteine

technical grade

W326305

L-cisteina

≥97%, FG

168149

L-cisteina

97%

95437

L-cisteina

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Astec CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (40:60:0.02, A:B:C)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

(200 μg /mL in 50:50 water:methanol)

Descrizione

Note legali

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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