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Key Documents

581508-U

Supelco

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 10 cm × 2.1 mm

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About This Item

Codice UNSPSC:
41115700
eCl@ss:
32110501
NACRES:
SB.52

Materiali

stainless steel column

Livello qualitativo

100

agenzia

suitable for USP L3

Nome Commerciale

Ascentis®

Caratteristiche

endcapped: no

Produttore/marchio commerciale

Ascentis®

Confezionamento

1 ea of

Parametri

≤70 °C temp. range
400 bar pressure (5801 psi)

tecniche

HPLC: suitable
LC/MS: suitable

Lungh. × D.I.

10 cm × 2.1 mm

Area superficiale

450 m2/g

Impurezze

<5 ppm metals

Matrice

fully porous particle
silica gel high purity, spherical

Gruppo funzionale matrice

silica phase

Dimensione particelle

5 μm

Dimensione pori

100 Å

operating pH range

2-6

applicazioni

food and beverages

Tecnica di separazione

hydrophilic interaction (HILIC)
normal phase

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Descrizione generale

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.

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Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificati d'analisi (COA)

Lot/Batch Number
270587-01
268839-01
263763-01
263761-01
261775-01

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Paweł Kubica et al.
Journal of chromatography. A, 1289, 13-18 (2013-04-04)
Two analytical procedures are proposed where HILIC and RPLC techniques are coupled with tandem mass spectrometry detection for rapid determination of trace amounts of nicotine in zero-level liquids for electronic cigarettes. Samples are prepared on the basis of the approach
Shigehisa Uchiyama et al.
Analytical chemistry, 80(9), 3285-3290 (2008-03-18)
A new method for the simultaneous determination of ozone and carbonyls in air using a two-bed cartridge system has been developed. Each bed consists of reagent-impregnated silica particles. The first contains trans-1,2-bis-(4-pyridyl) ethylene (BPE) while the second contains 2,4-dinitrophenylhydrazine (DNPH).
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 50(5), 703-709 (2009-06-30)
The performance of a narrow bore silica based monolith column (5 cm x 2 mm) was compared to 5 cm long narrow bore (internal diameter < or = 2.1 mm) columns, packed with shell particles (2.7 microm) and totally porous
Szabolcs Fekete et al.
Journal of pharmaceutical and biomedical analysis, 49(1), 64-71 (2008-11-29)
The performance of 5 cm long columns packed with shell particles was compared to totally porous sub-2 microm particles in gradient and isocratic elution separations of hormones (dienogest, finasteride, gestodene, levonorgestrel, estradiol, ethinylestradiol, noretistherone acetate, bicalutamide and tibolone). Peak capacities
Luana Magalhães et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1008, 98-107 (2015-12-08)
Nucleoside triphosphate diphosphohydrolase (NTPDase) is an enzyme belonging to the apyrase family that participates in the hydrolysis of the nucleosides di- and triphosphate to the corresponding nucleoside monophosphate. This enzyme underlies the virulence of parasites such as Leishmania. Recently, an
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Protocolli

Deriviatization, Hydrolysis, and HPLC Analysis of Amino Acids on SUPELCOSIL™ and ASCENTIS® Columns

Amino Acid Analysis is the suitable tool for precise determination of protein quantities but also provides detailed information regarding the relative amino.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.