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Y2146

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, and tryptophan

Sinonimo/i:

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements

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About This Item

Numero MDL:
MFCD01095679
Codice UNSPSC:
41106212
NACRES:
NA.85

Livello qualitativo

200

Forma fisica

powder

tecniche

transformation: suitable

applicazioni

microbiology

Temperatura di conservazione

room temp

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Applicazioni

Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Altre note

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Nota sulla preparazione

Dissolve 1.46 g of supplement per liter of medium.

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Pittogrammi

Corrosion
GHS05

Avvertenze

Danger

Indicazioni di pericolo

H318

Consigli di prudenza

P280 - P305 + P351 + P338

Classi di pericolo

Eye Dam. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Introduction to Yeast Media

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protocolli

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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