Y2021

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, tryptophan, and adenine

• Sinonimo/i: Yeast Synthetic Drop-out Media Supplements
• Numero MDL: MFCD01095679
• Codice UNSPSC: 41106212
• NACRES: NA.85

Proprietà

• Livello qualitativo: 200
• Forma fisica: powder
• tecniche: transformation: suitable
• applicazioni: microbiology
• Temperatura di conservazione: room temp

Descrizione

Applicazioni

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine has been used in yeast two-hybrid screening.

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Altre note

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Nota sulla preparazione

Dissolve 1.39 g of supplement per liter of medium.

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H318
• Consigli di prudenza: P280 - P305 + P351 + P338
• Classi di pericolo: Eye Dam. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable