Y1771

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

• Sinonimo/i: Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements
• Numero MDL: MFCD01095679
• Codice UNSPSC: 41106212
• NACRES: NA.85

Proprietà

• Livello qualitativo: 200
• Stato: powder
• tecniche: transformation: suitable
• applicazioni: microbiology
• Temperatura di conservazione: room temp

Descrizione

Applicazioni

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

• the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
• Yarrowia lipolytica PO1f strain positive transformants
• CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants

Altre note

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Nota sulla preparazione

Dissolve 1.46 g of supplement per liter of medium.

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H318
• Consigli di prudenza: P280 - P305 + P351 + P338
• Classi di pericolo: Eye Dam. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable