Y0771

Anti-phospho-YAP1 (pTyr³⁵⁷) antibody, Mouse monoclonal

clone PYP-76, purified from hybridoma cell culture

• Sinonimo/i: Anti-COB1, Anti-YAP, Anti-YAP-1, Anti-YAP2, Anti-YAP65, Anti-YKI
• Codice UNSPSC: 12352203
• NACRES: NA.44

Proprietà

• Origine biologica: mouse
• Coniugato: unconjugated
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: PYP-76, monoclonal
• Forma fisica: buffered aqueous solution
• PM: antigen ~65 kDa
• Reattività contro le specie: human
• tecniche: indirect ELISA: suitable; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using total cell extract of HEK-293T cells co-transfected with wt YAP and a constitutively active mutant of c-Abl (Δ1-81)
• Isotipo: IgM
• N° accesso UniProt: P46937
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: phosphorylation (pTyr357)
• Informazioni sul gene: human ... YAP1(10413) ; mouse ... Yap1(22601)

Descrizione

Descrizione generale

Monoclonal Anti-phospho-YAP1 (pTyr357) (mouse IgM isotype) is derived from the hybridoma PYP-76 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phosphopeptide corresponding to a fragment containing (pTyr357) of human YAP1 conjugated to KLH. Yes-associated protein 1 (YAP1) is a modular adapter protein with multiple protein interaction domains that was originally identified based on its interaction with the Src-family tyrosine kinase c-Yes. YAP1 contains a proline-rich amino terminus, a WW domain, a coiled-coil and a PDZ-binding motif at the extreme C-terminus.

Applicazioni

Anti-phospho-YAP1 (pTyr357) antibody, Mouse monoclonal has been used in

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunofluorescence

Azioni biochim/fisiol

YAP1 has been shown to stabilize p73 by preventing Itch-mediated ubiquitination of p73.

YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)