X4626

XTT sodium salt

powder, BioReagent, suitable for cell culture

• Sinonimo/i: 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt, 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt
• Formula empirica (notazione di Hill): C₂₂H₁₆N₇NaO₁₃S₂
• Numero CAS: 111072-31-2
• Peso molecolare: 673.52
• Numero MDL: MFCD00083517
• Codice UNSPSC: 12352207
• ID PubChem: 24902150

Proprietà

• Nome Commerciale: BioReagent
• Livello qualitativo: 200
• Saggio: ≥90%
• Forma fisica: powder
• Condizioni di stoccaggio: dry at room temperature
• tecniche: cell culture | mammalian: suitable; protein quantification: suitable
• applicazioni: detection
• Metodo di rivelazione: colorimetric
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: [Na+].COc1cc(c(cc1-n2nc(n[n+]2-c3cc(c(cc3OC)[N+]([O-])=O)S([O-])(=O)=O)C(=O)Nc4ccccc4)S([O-])(=O)=O)[N+]([O-])=O
• InChI: 1S/C22H17N7O13S2.Na/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);/q;+1/p-1; JACYMBNQPPWQML-UHFFFAOYSA-M

Descrizione

Descrizione generale

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Applicazioni

XTT sodium salt has been used to assess cell viability.

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines. The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.

Azioni biochim/fisiol

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

Principio

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 4.1A - Other explosive hazardous materials
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)