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V8379

Sigma-Aldrich

Vitronectin from human plasma

lyophilized powder, BioReagent, suitable for cell culture

Sinonimo/i:

Serum spreading factor

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About This Item

Numero CAS:
83380-82-9
Numero MDL:
MFCD00132575
Codice UNSPSC:
12352202
NACRES:
NA.75

Origine biologica

human plasma

Livello qualitativo

300

Nome Commerciale

BioReagent

Forma fisica

lyophilized powder

PM

A subunit 75 kDa
B subunit 65 kDa

Confezionamento

pkg of 50 μg

tecniche

cell culture | mammalian: suitable

Copertura della superficie

0.1 μg/cm2

Solubilità

water: soluble 0.050 mg/mL, clear, colorless

N° accesso UniProt

P04004

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Informazioni sul gene

human ... VTN(7448)

Categorie correlate

Applicazioni

Vitronectin can be used with cells with integrin receptors that bind vitronectin, including platelets, endothelial cells, melanoma cells, and osteosarcoma. It is recommended as a cell culture substratum at 0.1 μg/cm2. Optimal conditions for attachment depend on cell line and application.

Azioni biochim/fisiol

Vitronectin binds to glycosaminoglycans, is incorporated as an inhibitor to the membrane cytolytic attack complex of the complement system, has interaction with thrombin and antithrombin III during coagulation, and may play a role in the coagulation pathway.

Componenti

Vitronectin is a glycoprotein present in plasma and tissues, and is one of the major cell adhesion proteins, along with fibronectin, in plasma. The N-terminal residues of vitronectin are identical to somatomedin B and are followed by an R-G-D sequence that interacts with a specific cell-surface receptor. The central domain of vitronectin is enriched with hydrophobic residues. Closer to the C-terminus, it has an arginine rich region that allows for heparin binding activity.

Avvertenza

The lyophilized product should be stored desiccated at 2-8°C. Under these conditions it is stable for at least 2 years. Aliquots should be stored at -20°C or -70°C, but repeated freezing and thawing should be avoided.

Nota sulla preparazione

This product is supplied as a cell culture tested lyophilized powder from buffered saline. It is recommended to use polypropylene vessels during handling, due to the strong affinity of vitronectin for hydrophilic surfaces. Reconstitution of this product can be done with 1 mL of water per vial. The stock solution should then be filter-sterilized using a 0.2μm membrane and aliquoted and frozen for long term storage.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Theodora Chatzisideri et al.
Journal of medicinal chemistry, 65(1), 271-284 (2021-12-31)
c(RGDyK)-based conjugates of gemcitabine (GEM) with the carbonate and carbamate linkages in the 6-OH group of GEM were synthesized for the targeted delivery of GEM to integrin αvβ3, overexpressing cancer cells to increase the stability as well as the tumor
The receptor for urokinase-plasminogen activator (uPAR) controls plasticity of cancer cell movement in mesenchymal and amoeboid migration style.
Margheri F, Luciani C, Taddei ML, et al.
Oncotarget, 5(6), 1538-1553 (2014)
Francesca Posa et al.
Biomaterials, 267, 120484-120484 (2020-11-04)
Here we present the use of surface nanopatterning of covalently immobilized BMP-2 and integrin selective ligands to determine the specificity of their interactions in regulating cell adhesion and focal adhesion assembly. Gold nanoparticle arrays carrying single BMP-2 dimers are prepared
Lorenzo Sernissi et al.
Bioorganic & medicinal chemistry, 24(4), 703-711 (2016-01-13)
4-Amino- and 5-amino-cyclopropane pipecolic acids (CPAs) with cis relative stereochemistry between the carboxylic and amino groups were used as templates to prepare cyclic peptidomimetics containing the RGD sequence as possible integrin binders. The peptidomimetic c(RGD8) built on the 5-amino-CPA displayed
Ta-Chun Kao et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 102(3), 463-476 (2013-09-17)
Cancer-initiating cells [cancer stem cells (CSCs)] in colon cancer cells can be selectively suppressed when they are cultured on Pluronic (nanosegment)-grafted dishes, whereas CSCs are maintained on conventional tissue culture dishes and extracellular matrix-coated dishes. CSCs persist in tumors as
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