F1056
Human Fibronectin
from human plasma, powder, suitable for cell culture
Sinonimo/i:
Fibronectin
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About This Item
Prodotti consigliati
product name
Fibronectina, lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)
Origine biologica
human plasma
Livello qualitativo
Sterilità
sterile
Nome Commerciale
BioReagent
Saggio
≥95% (SDS-PAGE)
Forma fisica
lyophilized powder
Confezionamento
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
tecniche
cell culture | mammalian: suitable
Copertura della superficie
1‑5 μg/cm2
Solubilità
H2O: 1 mg/mL at 37 °C
N° accesso UniProt
Specificità del legame
Peptide Source: Collagen
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
Informazioni sul gene
human ... FN1(2335)
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Descrizione generale
Fibronectin is a class of high-molecular-weight adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The FN gene, which is highly conserved, gives rise to a minimum of 20 isoforms through alternative splicing in humans. These isoforms include notable variants such as cellular FN and plasma FN. Plasma FN is primarily synthesized in the liver, whereas cellular FN which represents only 5% of the circulating fibronectins is locally produced by various cell types.
Applicazioni
Fibronectin human plasma has been used:
- in neural selection medium to coat tissue culture surface for neuronal differentiation
- to coat culture vessels for characterization of iPSC and iPSC‑derived BMEC in 2D cell culture
- to coat culture supports for endothelial cell culture
Azioni biochim/fisiol
Fibronectin plays a major role in biological processes. It interacts with surface receptors and other major ECM proteins like collagen to mediate cellular signals. Fibronectin is involved in a variety of cellular functions, including adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation. This glycoprotein can bind to integrins, collagens, fibrins and glycosaminoglycans (GAGs). FN plays a crucial role in promoting cell cycle progression and proliferation. This interaction influences cyclins and immediate early response genes, contributing to cellular processes related to cell division and growth. FN is also involved in cell survival and protection from apoptosis in various cell types, including epithelial and endothelial cells and leukocytes. FN serves as a critical factor in promoting cell survival and preventing programmed cell death in these cellular contexts. Mutations in the FN gene are associated with FN glomerulopathy and spondylometaphyseal dysplasia. Fibronectin plays a significant role in the wound healing process, and in extracellular matrix (ECM) assembly, hence is considered suitable for tissue engineering applications.
Avvertenza
The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.
Nota sulla preparazione
This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.
Esclusione di responsabilità
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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Articoli
Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.
Protocolli
Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.
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