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S5171

Sigma-Aldrich

Human Fibronectin

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

Sinonimo/i:

sFN

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About This Item

Numero MDL:
MFCD00284706
Codice UNSPSC:
12352202
NACRES:
NA.75

Nome del prodotto

Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture

Origine biologica

human

Livello qualitativo

200

Ricombinante

expressed in E. coli

Nome Commerciale

BioReagent

Stato

solution

PM

~235-270 kDa

Confezionamento

pkg of 5 mg

tecniche

cell culture | mammalian: suitable

Copertura della superficie

1 μg/cm2

N° accesso UniProt

P02751

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

Informazioni sul gene

human ... FN1(2335)

Categorie correlate

Applicazioni

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

Componenti

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

Avvertenza

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

Nota sulla preparazione

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000115055
11180741
018M4061V
067M4133V
076M4047V

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N Okada et al.
The Journal of biological chemistry, 272(43), 26978-26984 (1997-10-27)
The pathogenic Gram-positive bacterium Streptococcus pyogenes (group A streptococcus) binds to fibronectin via protein F. In this study, we have investigated the binding properties of protein F to various multimeric tissue forms of fibronectin that appear on cell surfaces and
Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example
Giornale di Psichiatria e di Neuropatologia (2012)
Gentle cell handling with an ultra-slow instrument: creep-manipulation of cells
Thielecke H, et al.
Microscopica Acta, 11(11), 1230-1241 (2005)
Florian Weinberg et al.
International journal of molecular sciences, 21(23) (2020-12-03)
The epidermal growth factor receptor HER2 is overexpressed in 20% of breast cancer cases. HER2 is an orphan receptor that is activated ligand-independently by homodimerization. In addition, HER2 is able to heterodimerize with EGFR, HER3, and HER4. Heterodimerization has been
A Morla et al.
Nature, 367(6459), 193-196 (1994-01-13)
Fibronectin is an extracellular matrix protein that is important in development, wound healing and tumorigenesis. In the blood it is dimeric, but in tissues forms disulphide crosslinked fibrils. Here we show that a fragment from the first type-III repeat of

Articoli

Enhancing Lentiviral Transduction Efficiency

Enhancing Lentiviral Transduction Efficiency

Fibronectin

Fibronectin (FN) is a multifunctional, extracellular plasma glycoprotein produced by hepatocytes that circulates at near micromolar concentration and assembles into extracellular matrix fibrils at cell surfaces along with locally produced cellular FN.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolli

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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