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F1904

Sigma-Aldrich

Human Fibronectin

from human plasma, powder, purified 120 kDa α Chymotryptic Fragment (cell attachment region), suitable for cell culture

Sinonimo/i:

Fibronectin Fragment, Human Fibronectin

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About This Item

Codice UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.75

Nome del prodotto

Fibronectin 120 kDa α Chymotryptic Fragment (Cell Attachment region), Human purified,

Origine biologica

human

Livello qualitativo

Stato

lyophilized

PM

120 kDa

Produttore/marchio commerciale

Chemicon®

Concentrazione

1 mg/mL

tecniche

cell culture | mammalian: suitable

input

sample type induced pluripotent stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... FN1(2335)

Descrizione generale

PROPERTIES:

Contains the cell attachment region of the fibronectin molecule.
Product Source: Human plasma cryoprecipitate (plasma fibronectin), assayed negative for hepatitis B virus, hepatitis C Virus (HCV), HIV-1, HIV-2, HTLV-1, HTLV-2 and Treponema pallidum. Handle as if potentially infectious.

Applicazioni

F1904 is suitable for use in ELISA and affinity chromatography, and possibly molecular mapping of fibronectin function.

Stato fisico

Purified protein. Lyophilized in sodium citrate buffer, pH 7.4. Reconstitute with water.

Stoccaggio e stabilità

Maintain material at -20°C for 3 months or -70°C for six months.

Risultati analitici

One major band at 120kDa in SDS-PAGE

Note legali

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

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I clienti hanno visto anche

Slide 1 of 3

1 of 3

Fibronectin: purification, immunochemical properties, and biological activities.
Ruoslahti, E, et al.
Test, 82 Pt A, 803-831 (1982)
Location of the cell-attachment site in fibronectin with monoclonal antibodies and proteolytic fragments of the molecule.
Pierschbacher, M D, et al.
Cell, 26, 259-267 (1981)

Articoli

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocolli

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs at sigmaaldrich.com

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

Contenuto correlato

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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