T7818

Trizma^® Pre-set crystals

BioPerformance Certified, pH 7.5, average M_w 150.6

• Sinonimo/i: Tris crystals
• Codice UNSPSC: 12161700
• NACRES: NA.25

Proprietà

• Grado: BioPerformance Certified
• Forma fisica: crystalline powder
• PM: average M_w 150.6
• tecniche: cell culture | mammalian: suitable
• Impurezze: DNase, RNase, Protease, NICKase, none detected; endotoxin, tested; total aerobic microbial count, tested; ≤0.05 A₂₉₀; ≤0.5% water (Karl Fischer); ≤5 ppm Heavy metals (as Pb)
• pH: 7.50 (25 °C, 0.1 M)(lit.); 7.5
• Intervallo di pH utile: 7.0-9.0
• Solubilità: water: 0.66 g/mL, clear, colorless
• applicazioni: diagnostic assay manufacturing

Descrizione

Applicazioni

Dissolve in water to make a 0.1M solution. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Altre note

This product is a pre-mixed combination of Trizma base and Trizma HCl that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.

Nota sulla preparazione

Trizma solutions can be autoclaved.

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves