646563

DL-Dithiothreitol solution

1 M in H₂O

• Sinonimo/i: (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
• Formula empirica (notazione di Hill): C₄H₁₀O₂S₂
• Numero CAS: 3483-12-3
• Peso molecolare: 154.25
• Beilstein: 1719757
• Numero MDL: MFCD00004877
• Codice UNSPSC: 12161504
• ID PubChem: 24883472
• NACRES: NA.25

Proprietà

• Forma fisica: liquid
• Impiego in reazioni chimiche: reagent type: reductant
• Concentrazione: 1 M in H₂O
• Densità: 1.04 g/mL at 20 °C; 1.035 g/mL at 25 °C
• Stringa SMILE: O[C@H](CS)[C@H](O)CS
• InChI: 1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1; VHJLVAABSRFDPM-QWWZWVQMSA-N

Descrizione

Descrizione generale

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.

Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Applicazioni

DL-Dithiothreitol solution has been used:

• in western blot analysis
• as a supplement in lysis buffer to study its effects on sperm lysis
• as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
• in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Caratteristiche e vantaggi

Low toxicity and reduced odor compared to other thiol compounds.

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H318
• Consigli di prudenza: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P362 + P364
• Classi di pericolo: Eye Dam. 1 - Skin Irrit. 2
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter