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646563

Supelco

DL-Dithiothreitol solution

1 M in H2O

Sinonimo/i:

(±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT

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About This Item

Formula empirica (notazione di Hill):
C4H10O2S2
Numero CAS:
3483-12-3
Peso molecolare:
154.25
Beilstein:
1719757
Numero MDL:
MFCD00004877
Codice UNSPSC:
12161504
ID PubChem:
24883472
NACRES:
NA.25

Forma fisica

liquid

Livello qualitativo

100

Impiego in reazioni chimiche

reagent type: reductant

Concentrazione

1 M in H2O

Densità

1.04 g/mL at 20 °C
1.035 g/mL at 25 °C

Stringa SMILE

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
VHJLVAABSRFDPM-QWWZWVQMSA-N

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Descrizione generale

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Applicazioni

DL-Dithiothreitol solution has been used:
  • in western blot analysis
  • as a supplement in lysis buffer to study its effects on sperm lysis
  • as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
  • in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Caratteristiche e vantaggi

Low toxicity and reduced odor compared to other thiol compounds.

Accessorio

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Corrosion
GHS05

Avvertenze

Danger

Indicazioni di pericolo

H315,H318

Classi di pericolo

Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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L-(−)-Dithiothreitol ≥95% (titration)

Sigma-Aldrich

D9760

L-(−)-Dithiothreitol

Iodoacetamide Single use vial of 56 mg

Sigma-Aldrich

A3221

Iodoacetamide

Sputolysin® Reagent Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

Millipore

560000

Sputolysin® Reagent

DTT OmniPur® Grade, >= 99.4%, Used for reduction of protein disulfide linkages

Millipore

3860-OP

DTT

Calista K L Ng et al.
Human molecular genetics, 24(11), 3163-3171 (2015-02-26)
mRNA decay is an essential and active process that allows cells to continuously adapt gene expression to internal and environmental cues. There are two mRNA degradation pathways: 3' to 5' and 5' to 3'. The DCPS protein is the scavenger
Yukari Yamane-Sando et al.
MicrobiologyOpen, 3(2), 196-212 (2014-02-11)
Sphingolipids are a family of eukaryotic lipids biosynthesized from sphingoid long-chain bases (LCBs). Sphingolipids are an essential class of lipids, as their depletion results in cell death. However, acute LCB supplementation is also toxic; thus, proper cellular LCB levels should
I Dzneladze et al.
Leukemia, 29(7), 1485-1495 (2015-03-05)
In this study, we investigated the role of inositol polyphosphate-4-phosphatase, type-II (INPP4B) in acute myeloid leukemia (AML). We observed that AML patients with high levels of INPP4B (INPP4B(high)) had poor response to induction therapy, shorter event-free survival and shorter overall
Kazuya Ishikawa et al.
Nature communications, 5, 5430-5430 (2014-11-13)
Pathogen effector proteins are delivered to host cells to suppress plant immunity. However, the mechanisms by which effector proteins function are largely unknown. Here we show that expression of XopP(Xoo), an effector of rice pathogen Xanthomonas oryzae pv. oryzae, in
Boseon Kim et al.
The EMBO journal, 34(13), 1801-1815 (2015-05-17)
Terminal uridylyl transferases (TUTs) function as integral regulators of microRNA (miRNA) biogenesis. Using biochemistry, single-molecule, and deep sequencing techniques, we here investigate the mechanism by which human TUT7 (also known as ZCCHC6) recognizes and uridylates precursor miRNAs (pre-miRNAs) in the
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