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Key Documents

T3069

Sigma-Aldrich

Trizma® hydrochloride solution

pH 8.0, BioPerformance Certified, 2 M, suitable for cell culture

Sinonimo/i:

Tris hydrochloride solution

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About This Item

Codice UNSPSC:
12161700
NACRES:
NA.25

Grado

BioPerformance Certified
for molecular biology

Livello qualitativo

200

Forma fisica

solution

Concentrazione

2 M

tecniche

cell culture | mammalian: suitable

Impurezze

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

8.0

Intervallo di pH utile

7.0-9.0

Assorbimento

≤0.05 at 290 at 40%

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Descrizione generale

Tris(hydroxymethyl)aminomethane (Tris) has a pH buffering range between 7.0 - 9.0 and is a routine buffer in biological sciences. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. It displays negligible binding towards metal in solutions.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Applicazioni

Trizma® hydrochloride solution has been used as a component of lysis buffer for testing on commercially available biological sample preservation paper types. It has also been used as a vehicle and for dissolving leptin in reporter gene assay studies.

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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(Un) suitability of the use of pH buffers in biological, biochemical and environmental studies and their interaction with metal ions-a review
Ferreira CMH, et al.
Royal Society of Chemistry Advances, 5(39) (5)
Optimal DNA extractions from blood on preservation paper limits conservation genomic but not conservation genetic applications
Stowell SML, et al.
Journal for nature conservation, 46 (46)
Universal buffers for use in biochemistry and biophysical experiments
Brooke D, et al.
AIMS Biophysics, 2(3) (2)
Elke Ericson et al.
Molecular and cellular endocrinology, 412, 257-264 (2015-05-20)
In a world with increasing incidences of obesity, it becomes critical to understand the detailed regulation of appetite. To identify novel regulators of the signaling mediated by one of the key hormones of energy homeostasis, leptin, we screened a set

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