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Key Documents

T2819

Sigma-Aldrich

Trizma® hydrochloride solution

pH 9.0, BioPerformance Certified, 1 M, suitable for cell culture

Sinonimo/i:

Tris hydrochloride solution

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About This Item

Codice UNSPSC:
12161700
NACRES:
NA.25

Grado

BioPerformance Certified
for molecular biology

Livello qualitativo

200

Forma fisica

solution

Concentrazione

1 M

tecniche

cell culture | mammalian: suitable

Impurezze

DNase, RNase, Protease, none detected
 bioburden, tested
 endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

9.0

Intervallo di pH utile

7.0-9.0

Assorbimento

≤0.05 at 290 at 40%

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Descrizione generale

Tris hydrochloride is used to prepare buffer, mainly at a physiological range of 7.3 to 7.5. This buffer is well-suited with biological fluids. It is used in laboratories as primary pH standard in clinical laboratories.
A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Applicazioni

Trizma® hydrochloride solution has been used:
  • to neutralize IgG bound to protein A/G column
  • as a component in BigDye buffer for sequencing reaction
  • to block slides

Note legali

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Experimental protocol for detecting cyanobacteria in liquid and solid samples with an antibody microarray chip
Blanco Y, et al.
Journal of Visualized Experiments, 142(120), e54994-e54994 (2017)
Using improved technology for filter paper-based blood collection to survey wild Sika deer for antibodies to hepatitis E virus
Yu C, et al.
Journal of Virological Methods, 142(1-2), 143-150 (2007)
Genotypic inference of HIV-1 tropism using population-based sequencing of V3
McGovern RA, et al.
Journal of Visualized Experiments, 142(46), e2531-e2531 (2010)
Tris/Tris (textperiodcentered) HCl: a standard buffer for use in the physiologic pH range
Durst RA and Staples BR
Clinical Chemistry, 18(3), 206-208 (1972)
Hao Wang et al.
Plant physiology, 172(2), 1003-1018 (2016-08-18)
Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway

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