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Merck
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Documenti fondamentali

SRP3268

Sigma-Aldrich

PDGF-AA human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Sinonimo/i:

GDGF, ODGF

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Origine biologica

human

Ricombinante

expressed in E. coli

Saggio

≥98% (HPLC)
≥98% (SDS-PAGE)

Stato

lyophilized

Potenza

1.0-3.0 ng/mL ED50

PM

28.5 kDa

Confezionamento

pkg of 10 μg

Impurezze

<0.1 EU/μg endotoxin, tested

Colore

white to off-white

N° accesso UniProt

P04085

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... PDGFA(5154)

Descrizione generale

Platelet derived growth factors (PDGFs) are disulfide-linked glycoprotein dimers consisting of two 12.0-13.5kDa polypeptide chains, designated as PDGF-A and PDGF-B chains. There are three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kDa disulfide-linked homodimer of two A chains (250 total amino acids).

Azioni biochim/fisiol

The platelet derived growth factors (PDGFs) are stored in platelet a-granules and are released upon platelet activation. They are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. These proteins are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells.

Stato fisico

Lyophilized from 10 mM Acetic Acid.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H319

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0212AFC03
0510AFC03

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Characterization of the G-quadruplexes in the duplex nuclease hypersensitive element of the PDGF-A promoter and modulation of PDGF-A promoter activity by TMPyP4.
Qin Y
Nucleic Acids Research, 35(22), 7698-7713 (2007)
The use of autologous blood-derived growth factors in bone regeneration.
Civinini R
Clinical Cases in Mineral and Bone Metabolism : The Official Journal of the Italian Society of Osteoporosis, Mineral Metabolism, and Skeletal Diseases, 8(1), 25-31 (2011)
Brashket Seth et al.
Neurotoxicology, 70, 161-179 (2018-11-25)
During the mammalian brain development, oligodendrocyte progenitor cells (OPCs) are generated from neuroepithelium and migrate throughout the brain. Myelination is a tightly regulated process which involves time framed sequential events of OPCs proliferation, migration, differentiation and interaction with axons for
Structural and functional studies on platelet-derived growth factor.
C H Heldin
The EMBO journal, 11(12), 4251-4259 (1992-12-01)
The biology of platelet-derived growth factor.
R Ross et al.
Cell, 46(2), 155-169 (1986-07-18)

Articoli

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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